Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies: utility for detection of diverse HIV-1 subtypes.
Pubmed ID: 17019363
Pubmed Central ID: PMC2365716
Journal: Journal of acquired immune deficiency syndromes (1999)
Publication Date: Nov. 1, 2006
Affiliation: Division of Viral Products, Center for Biologics Evaluation and Research, FDA, Bethesda, MD 20892, USA.
MeSH Terms: Humans, HIV Infections, HIV-1, Sensitivity and Specificity, Diagnosis, Differential, HIV Seropositivity, AIDS Serodiagnosis, False Positive Reactions, Cross Reactions, AIDS Vaccines, HIV Antibodies, Peptide Library
Grants: AI-51231, AI36085, AI47053, BY1-HB-5026-01, HD-40125, MH-766767, Z99 AI999999, R01 AI051231, R01 AI036085, R01 HD040125, R01 AI047053, R37 AI051231
Authors: Busch MP, Todd D, Khurana S, Needham J, Park S, Mathieson B, Nemo G, Nyambi P, Zolla-Pazner S, Laal S, Mulenga J, Chomba E, Hunter E, Allen S, McIntyre J, Hewlett I, Lee S, Tang S, Cowan E, Beyrer C, Altfeld M, Yu XG, Tounkara A, Koita O, Kamali A, Nguyen N, Graham BS, Mugenyi P, Anzala O, Sanders E, Ketter N, Fast P, Golding H
Cite As: Khurana S, Needham J, Park S, Mathieson B, Busch MP, Nemo G, Nyambi P, Zolla-Pazner S, Laal S, Mulenga J, Chomba E, Hunter E, Allen S, McIntyre J, Hewlett I, Lee S, Tang S, Cowan E, Beyrer C, Altfeld M, Yu XG, Tounkara A, Koita O, Kamali A, Nguyen N, Graham BS, Todd D, Mugenyi P, Anzala O, Sanders E, Ketter N, Fast P, Golding H. Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies: utility for detection of diverse HIV-1 subtypes. J Acquir Immune Defic Syndr 2006 Nov 1;43(3):304-12.
Studies:
- Retrovirus Epidemiology Donor Study (REDS) Allogeneic Donor and Recipient Repository (RADAR)
- Retrovirus Epidemiology Donor Study (REDS) General Leukocyte/Plasma Repository (GLPR)
- Retrovirus Epidemiology Donor Study (REDS) HTLV Cohort (HTLV)
- Retrovirus Epidemiology Donor Study (REDS) Human Herpes Virus 8 Special Collection from the General Leukocyte/Plasma Repository (HHV8)
- Retrovirus Epidemiology Donor Study (REDS): Special Repository Collections (SR)
- Retrovirus Epidemiology Donor Study-II (REDS II) Donation and Deferral Database (CORE)
- Retrovirus Epidemiology Donor Study-II (REDS II) Donor Iron Status Evaluation Study (RISE)
- Retrovirus Epidemiology Donor Study-II (REDS II) Leukocyte Antibodies Prevalence Study (LAPS)
- Retrovirus Epidemiology Donor Study-II (REDS II) Molecular Surveillance (MS)
Abstract
Because increasing numbers of HIV vaccine candidates are being tested globally, it is essential to differentiate vaccine- from virus-induced antibodies. Most of the currently tested vaccines contain multiple viral components. As a result, many vaccine recipients give positive results in FDA-licensed HIV serodetection tests. We have identified conserved sequences in Env-gp41 and Gag-p6, which are recognized soon after infection but are not included in most HIV vaccine candidates. A new HIV serodetection assay, the HIV-SELECTEST, was established that distinguishes between vaccine-induced antibodies and seroconversion due to true HIV infections. It is important to make this assay globally relevant, because many clinical trials are conducted around the world where most HIV infections are due to non-B subtype HIV-1. Therefore, the current study examined the reactivity of plasma samples from >3,000 infections with diverse HIV subtypes worldwide. The HIV-SELECTEST performed at >99% specificity and sensitivity. Both recent and established infections with clades A, B, C, D, E, F, G, J, and CRFs were detected. Antibodies elicited by other vaccinations or infections endemic to the clinical trial sites did not react in this assay. Therefore, HIV-SELECTEST could be an important differential diagnostic tool for HIV vaccine trials, blood banks, and population screening worldwide.