Transfusion-Transmitted Viruses Study (TTVS)

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Accession Number
HLB01910909a

Study Type
Epidemiology Study

Collection Type
Open BioLINCC Study See bottom of this webpage for request information

Study Period
1974-1980

NHLBI Division
DBDR

Clinical Trial URLs
N/A

Primary Publication URLs
N/A

Related Studies
NANB-TAH

Consent

Commercial Use Specimen Restrictions No

Non-Genetic Use Specimen Restrictions Based On Area Of Use Yes

Genetic Use Of Specimens Allowed? Yes

Genetic Use Area Of Research Restrictions Yes

Specific Consent Restrictions
Consent for both genetic and non-genetic use of biospecimens is restricted to studies involving transfusion safety.

Objectives

To establish a repository of specimens collected from prospectively identified cases of non-A, non-B (NANB) hepatitis after blood transfusion. The major intentions were to determine the incidence of that occurrence, identify the characteristics of the donors associated with the event, and have a resource available to compare laboratory donor screening methods during the study and in subsequent years following completion of the study.

Background

From July 1974 through June 1980, the TTVS group prospectively identified cases of NANB hepatitis in a cohort of 1,533 transfused patients hospitalized short-term for various routine operative procedures. The TTVS collection has been used in a variety of studies, such as the evaluation and validation of Hepatitis C Virus (HCV) immunoassays in screening blood donors (1). In addition, specimens have been requested to evaluate poorly characterized hepatotropic viruses, transmissibility of new viruses (hepatitis G, HHV-8) by transfusion, and the dynamics of HBV/HCV viral replication and seroconversion in recipients.

Participants

Serum specimens from 5,655 blood donors were collected from 4 participating blood centers distributed across the US. 3,121 patients were consented to participate in a study of viral hepatitis after blood transfusion. The majority of patients were scheduled for elective surgery. Of these subjects, 1,533 subsequently received blood transfusions. Both transfusion recipients and control subjects that did not require transfusion were observed and blood was collected prior to surgery or pre-transfusion and again during visits at 2-3 week intervals during the first 6 months following the transfusion episode and again between 8-10 months.

A subset of this cohort was subsequently recruited into the Natural History Study of Non-A, Non-B Post Transfusion Hepatitis (NANB-TAH) that also has data and biospecimens available. Subjects cannot be linked between these two studies.

Design

The collection originally consisted of aliquots of serum acquired at patient observation visits and from the collection agency for every donor to each TTVS recipient. These sera included the pre-transfusion or pre-surgical specimen, all interval blood draws from these patients and the non-transfused control patients, a specimen from the donor at the time of blood collection and in a subpopulation of donors a follow-up specimen from donors implicated in suspected cases of transfusion transmitted hepatitis and a matched set of non-implicated donors.

Conclusions

The TTVS collection provides sera from a large transfusion donor-recipient study in which an untransfused control population with reasonably matched characteristics and of adequate size is available.

Publications


  1. Mosley JW, et al. (1990) Non-A, non-B hepatitis and antibody to hepatitis C virus. JAMA 263(1):77-78.

Additional Details

Please note that biospecimen availability is subject to review by the NHLBI, BioLINCC, and the NHLBI Biorepository. Certain biospecimens may not be made available for your request. Section 3 of the BioLINCC handbook describes the components of the review process

Visits (Vials):

Updated 6th November 2025.

 

Serum

Plasma or Serum

Total

Recipient Visit 1

9,846

993

10,839

Recipient Visit 2

5,464

21

5,485

Recipient Visit 3

11,395

33

11,428

Recipient Visit 4

10,118

88

10,206

Recipient Visit 5

5,958

296

6,254

Recipient Visit 6

8,136

778

8,914

Recipient Visit 7

4,977

203

5,180

Recipient Visit 8

5,895

289

6,184

Recipient Visit 9

7,955

700

8,655

Recipient Visit 10

5,491

248

5,739

Recipient Visit 11

6,379

309

6,688

Recipient Visit 12

6,657

595

7,252

Recipient Visit 13

3,547

170

3,717

Recipient Visit 14

1,757

51

1,808

Recipient Visit 15

1,047

28

1,075

Recipient Visit 16

758

20

778

Recipient Visit 17

673

13

686

Recipient Visit 18

549

14

563

Recipient Visit 19

507

8

515

Recipient Visit 20

405

7

412

Recipient Visit 21

327

3

330

Recipient Visit 22

247

5

252

Recipient Visit 23

193

0

193

Recipient Visit 24

198

2

200

Recipient Visit 25

155

1

156

Recipient Visit 26

95

0

95

Recipient Visit 27

83

0

83

Recipient Visit 28

79

0

79

Recipient Visit 29

80

0

80

Recipient Visit 30

59

0

59

Recipient Visit 31

60

0

60

Recipient Visit 32

42

0

42

Recipient Visit 33

30

0

30

Recipient Visit 34

30

0

30

Recipient Visit 35

28

0

28

Recipient Visit 36

23

0

23

Recipient Visit 37

21

0

21

Recipient Visit 38

12

0

12

Recipient Visit 39

26

0

26

Recipient Visit 40

21

0

21

Recipient Visit 41

13

0

13

Recipient Visit 42

14

0

14

Recipient Visit 43

18

0

18

Donor 1

11,350

1,302

12,652

Donor 2

9,504

1,036

10,540

Donor 3

6,108

599

6,707

Donor 4

4,684

423

5,107

Donor 5

3,294

297

3,591

Donor 6

2,410

217

2,627

Donor 7

1,613

147

1,760

Donor 8

1,077

107

1,184

Donor 9

711

68

779

Donor 10

450

35

485

Donor 11

323

33

356

Donor 12

207

20

227

Donor 13

115

11

126

Donor 14

69

4

73

Donor 15

14

3

17

Donor 17

1

0

1

 

Visits (Subjects):

Updated 6th November 2025.

 

Serum

Total number of subjects

Average volume (ml) per subject

Recipient Visit 1

1,492

3.22

Recipient Visit 2

1,263

4.22

Recipient Visit 3

1,499

3.54

Recipient Visit 4

1,444

3.70

Recipient Visit 5

1,285

4.02

Recipient Visit 6

1,404

3.76

Recipient Visit 7

1,234

4.21

Recipient Visit 8

1,295

4.13

Recipient Visit 9

1,401

3.74

Recipient Visit 10

1,232

4.21

Recipient Visit 11

1,253

3.97

Recipient Visit 12

1,202

3.69

Recipient Visit 13

853

4.07

Recipient Visit 14

370

3.88

Recipient Visit 15

199

3.64

Recipient Visit 16

141

3.67

Recipient Visit 17

114

3.53

Recipient Visit 18

96

3.45

Recipient Visit 19

84

3.69

Recipient Visit 20

69

3.79

Recipient Visit 21

61

3.56

Recipient Visit 22

42

3.50

Recipient Visit 23

38

3.40

Recipient Visit 24

35

3.44

Recipient Visit 25

27

3.22

Recipient Visit 26

22

2.39

Recipient Visit 27

20

3.06

Recipient Visit 28

15

2.91

Recipient Visit 29

14

2.68

Recipient Visit 30

13

2.68

Recipient Visit 31

12

2.71

Recipient Visit 32

8

2.63

Recipient Visit 33

6

2.71

Recipient Visit 34

5

2.84

Recipient Visit 35

4

2.86

Recipient Visit 36

3

3.37

Recipient Visit 37

3

3.18

Recipient Visit 38

3

2.50

Recipient Visit 39

3

2.12

Recipient Visit 40

3

2.50

Recipient Visit 41

2

3.88

Recipient Visit 42

3

1.92

Recipient Visit 43

2

3.38

Donor 1

1,512

4.31

Donor 2

1,246

4.40

Donor 3

816

4.14

Donor 4

606

4.23

Donor 5

446

4.12

Donor 6

327

4.01

Donor 7

227

3.99

Donor 8

152

3.94

Donor 9

104

3.87

Donor 10

68

3.93

Donor 11

49

3.99

Donor 12

30

4.04

Donor 13

16

3.89

Donor 14

10

3.51

Donor 15

3

4.17

Donor 17

1

2.50

 

 

Plasma or Serum

Total number of subjects

Average volume (ml) per subject

Recipient Visit 1

985

0.22

Recipient Visit 2

21

0.25

Recipient Visit 3

32

0.26

Recipient Visit 4

85

0.24

Recipient Visit 5

293

0.25

Recipient Visit 6

773

0.26

Recipient Visit 7

203

0.25

Recipient Visit 8

289

0.25

Recipient Visit 9

696

0.26

Recipient Visit 10

247

0.24

Recipient Visit 11

305

0.24

Recipient Visit 12

591

0.24

Recipient Visit 13

165

0.23

Recipient Visit 14

49

0.24

Recipient Visit 15

27

0.22

Recipient Visit 16

19

0.21

Recipient Visit 17

13

0.21

Recipient Visit 18

14

0.19

Recipient Visit 19

8

0.18

Recipient Visit 20

7

0.19

Recipient Visit 21

3

0.20

Recipient Visit 22

5

0.17

Recipient Visit 24

2

0.15

Recipient Visit 25

1

0.05

Donor 1

939

0.23

Donor 2

770

0.23

Donor 3

474

0.23

Donor 4

334

0.23

Donor 5

253

0.22

Donor 6

180

0.23

Donor 7

126

0.23

Donor 8

90

0.21

Donor 9

58

0.21

Donor 10

32

0.20

Donor 11

31

0.21

Donor 12

18

0.22

Donor 13

11

0.20

Donor 14

4

0.24

Donor 15

3

0.23


 

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Resources Available

Specimens Only

Study Catalog

Study Publications (20)

Materials Available

Study Documents

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