Reduced thrombosis in Klkb1-/- mice is mediated by increased Mas receptor, prostacyclin, Sirt1, and KLF4 and decreased tissue factor.

Pubmed ID: 25339356

Pubmed Central ID: PMC4304115

Journal: Blood

Publication Date: Jan. 22, 2015

Affiliation: Divisions of Hematology and Oncology, Department of Medicine, Case Western Reserve University, Cleveland, OH; Department of Pathology, Case Western Reserve University, Cleveland, OH;

MeSH Terms: Nerve Tissue Proteins, Animals, RNA, Messenger, Peptide Fragments, Mice, Angiotensin II, Epoprostenol, Mice, Knockout, Proto-Oncogene Proteins, Receptor, Bradykinin B2, Receptors, G-Protein-Coupled, Sulfonamides, Kruppel-Like Transcription Factors, Imidazoles, Prekallikrein, Thromboplastin, Carotid Artery Thrombosis, Naphthalenes, Partial Thromboplastin Time, Pyrones, Sirtuin 1, Synaptotagmins, Proto-Oncogene Mas, Kruppel-Like Factor 4

Grants: F32 DK093226, R21 HL112666, R01 HL052779, 1S10RR031845, F32DK093226, HL052779-18, HL112666-2, S10 RR031845

Authors: Fang C, Grobe N, Schmaier AH, Merkulova A, Stavrou EX, Alhalabi O, Antoniak S, Mackman N

Cite As: Stavrou EX, Fang C, Merkulova A, Alhalabi O, Grobe N, Antoniak S, Mackman N, Schmaier AH. Reduced thrombosis in Klkb1-/- mice is mediated by increased Mas receptor, prostacyclin, Sirt1, and KLF4 and decreased tissue factor. Blood 2015 Jan 22;125(4):710-9. Epub 2014 Oct 22.

Studies:

Abstract

The precise mechanism for reduced thrombosis in prekallikrein null mice (Klkb1(-/-)) is unknown. Klkb1(-/-) mice have delayed carotid artery occlusion times on the rose bengal and ferric chloride thrombosis models. Klkb1(-/-) plasmas have long-activated partial thromboplastin times and defective contact activation-induced thrombin generation that partially corrects upon prolonged incubation. However, in contact activation-induced pulmonary thromboembolism by collagen/epinephrine or long-chain polyphosphate, Klkb1(-/-) mice, unlike F12(-/-) mice, do not have survival advantage. Klkb1(-/-) mice have reduced plasma BK levels and renal B2R mRNA. They also have increased expression of the renal receptor Mas and plasma prostacyclin. Increased prostacyclin is associated with elevated aortic vasculoprotective transcription factors Sirt1 and KLF4. Treatment of Klkb1(-/-) mice with the Mas antagonist A-779, COX-2 inhibitor nimesulide, or Sirt1 inhibitor splitomicin lowers plasma prostacyclin and normalizes arterial thrombosis times. Treatment of normal mice with the Mas agonist AVE0991 reduces thrombosis. Klkb1(-/-) mice have reduced aortic tissue factor (TF) mRNA, antigen, and activity. In sum, Klkb1(-/-) mice have a novel mechanism for thrombosis protection in addition to reduced contact activation. This pathway arises when bradykinin delivery to vasculature is compromised and mediated by increased receptor Mas, prostacyclin, Sirt1, and KLF4, leading to reduced vascular TF.