African Ancestry Analysis and Admixture Genetic Mapping for Proliferative Diabetic Retinopathy in African Americans.

Pubmed ID: 26098467

Pubmed Central ID: PMC4477259

Journal: Investigative ophthalmology & visual science

Publication Date: June 1, 2015

Affiliation: Department of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States.

MeSH Terms: Humans, Male, Adult, Female, Aged, Case-Control Studies, Odds Ratio, Risk Factors, Genetic Predisposition to Disease, Middle Aged, Genome-Wide Association Study, Genotype, Regression Analysis, Blood Pressure, Diabetes Mellitus, Type 2, Chromosome Mapping, Diabetic Retinopathy, Black or African American, Glycated Hemoglobin

Grants: UL1 TR000124, Z99 EY999999, N01HC65226, P30 DK063491, K12 EY016335, K12-EY16335, ZIA EY000403-12, ZIA EY000426-09, Z01 EY000426-04, ZIA EY000426-13, Z01 EY000425-04, ZIA EY000426-11, ZIA EY000403-15, ZIA EY000426-08, ZIA EY000403-10, ZIA EY000403-13, ZIA EY000426-12, Z01 EY000403-07, ZIA EY000426-07, ZIA EY000403-14, ZIA EY000403-09, Z01 EY000426-05, ZIA EY000425-06, ZIA EY000426-06, ZIA EY000403-08, ZIA EY000403-11, ZIA EY000426-10, Z01 EY000403-06

Authors: Li X, Reich D, James M, Rotter JI, Tandon A, Chen CJ, Penman A, Hancock H, Husain D, Andreoli C, Kuo JZ, Idowu O, Riche D, Papavasilieou E, Brauner S, Smith SO, Hoadley S, Richardson C, Kieser T, Vazquez V, Chi C, Fernandez M, Harden M, Cotch MF, Siscovick D, Taylor HA, Wilson JG, Wong TY, Klein R, Klein BE, Patterson N, Sobrin L

Cite As: Tandon A, Chen CJ, Penman A, Hancock H, James M, Husain D, Andreoli C, Li X, Kuo JZ, Idowu O, Riche D, Papavasilieou E, Brauner S, Smith SO, Hoadley S, Richardson C, Kieser T, Vazquez V, Chi C, Fernandez M, Harden M, Cotch MF, Siscovick D, Taylor HA, Wilson JG, Reich D, Wong TY, Klein R, Klein BE, Rotter JI, Patterson N, Sobrin L. African Ancestry Analysis and Admixture Genetic Mapping for Proliferative Diabetic Retinopathy in African Americans. Invest Ophthalmol Vis Sci 2015 Jun;56(6):3999-4005.

Studies:

Abstract

PURPOSE: To examine the relationship between proportion of African ancestry (PAA) and proliferative diabetic retinopathy (PDR) and to identify genetic loci associated with PDR using admixture mapping in African Americans with type 2 diabetes (T2D). METHODS: Between 1993 and 2013, 1440 participants enrolled in four different studies had fundus photographs graded using the Early Treatment Diabetic Retinopathy Study scale. Cases (n = 305) had PDR while controls (n = 1135) had nonproliferative diabetic retinopathy (DR) or no DR. Covariates included diabetes duration, hemoglobin A1C, systolic blood pressure, income, and education. Genotyping was performed on the Affymetrix platform. The association between PAA and PDR was evaluated using logistic regression. Genome-wide admixture scanning was performed using ANCESTRYMAP software. RESULTS: In the univariate analysis, PDR was associated with increased PAA (odds ratio [OR] = 1.36, 95% confidence interval [CI] = 1.16-1.59, P = 0.0002). In multivariate regression adjusting for traditional DR risk factors, income and education, the association between PAA and PDR was attenuated and no longer significant (OR = 1.21, 95% CI = 0.59-2.47, P = 0.61). For the admixture analyses, the maximum genome-wide score was 1.44 on chromosome 1. CONCLUSIONS: In this largest study of PDR in African Americans with T2D to date, an association between PAA and PDR is not present after adjustment for clinical, demographic, and socioeconomic factors. No genome-wide significant locus (defined as having a locus-genome statistic > 5) was identified with admixture analysis. Further analyses with even larger sample sizes are needed to definitively assess if any admixture signal for DR is present.